Microscope Activity.doc x712d

200 Microscopy: Parts & Observations 9 Name: ______________________

Date: ___________

1. Label the parts of a microscope on the diagram below.

Arm Base Body tube Coarse adjustment Diaphragm

1

WORD BANK Eyepiece Fine adjustment High power objective Light source Low power objective

Nosepiece Stage Stage clips Med. Power objective

Minority Science Programs – School of Biological Sciences – University of California, Irvine

2. Match the part of the microscope with its function. ___ ___

Where the slide is placed Used to focus when using the low power objective

1. Objectives 2. Base

___

Magnifies the specimen

3. Arm

___ Used to carry microscope adjustment ___ Used to focus when using the high power objective

4. Course

___

6. Eyepiece

The lens you look through, magnifies the specimen

___ s the microscope adjustment

3. Magnification:

5. Stage 7. Fine

Calculate the total magnification for each objective.

Eyepiece: 10X

Objectives: 4X, 10X, or 40X

4X objective:

__ ___ x ____ __ = _____ X__

10X objective: ______ x ______ = _____X__ 40X objective: ______ x ______ = _____X__

4. Observe the letter “e” with your naked eye and the microscope. Draw only what you see through the eyepiece. Include the correct magnification.

Naked eye

_______ X

What do you observe about the orientation and direction of the image? ___________________________________________________________________________ ___________________________________________________________________________ ___________________________________________________________________________ 5. Observe the colored comic strip with your naked eye and the microscope. Draw only what you see through the eyepiece. Include the correct magnification.

Naked eye

_______ X

6. Observe a prepared slide at different magnifications. Record the title of the slide. Draw only what you see through the eyepiece. Include the correct magnification. Slide title: __________________________

_______ X

_______ X

Slide Preparations: Letter “e”: Make a class set of letter “e” slides by printing out a page of tabdelimited e’s. Cut out each letter and secure them to slides using clear nail polish and a cover slip. Let dry overnight before using. Comic strip: Cut out small pieces of colored newspaper comic strips. Have students place them on a slide with a cover slip when viewing under the microscope. Prepared slides: Use a commercially available set of assorted prepared slides. Cheek cells: Have each student scrape the inside of their cheek using a cotton swab. Smear the swab on a clean slide to dislodge the cells. Add a drop of bromothymol blue stain and cover with a cover slip. Disinfect used slides with bleach. Only allow students to handle their own cheek cell slides.